Immobilization of chitosan film containing semaphorin 3A onto a microarc oxidized titanium implant surface via silane reaction to improve MG63 osteogenic differentiation
Identifieur interne : 001A03 ( Main/Exploration ); précédent : 001A02; suivant : 001A04Immobilization of chitosan film containing semaphorin 3A onto a microarc oxidized titanium implant surface via silane reaction to improve MG63 osteogenic differentiation
Auteurs : Kaixiu Fang [République populaire de Chine] ; Wen Song [République populaire de Chine] ; Lifeng Wang [République populaire de Chine] ; Sen Jia [République populaire de Chine] ; Hongbo Wei [République populaire de Chine] ; Shuai Ren [République populaire de Chine] ; Xiaoru Xu [République populaire de Chine] ; Yingliang Song [République populaire de Chine]Source :
- International Journal of Nanomedicine [ 1176-9114 ] ; 2014.
Abstract
Improving osseointegration of extensively used titanium (Ti) implants still remains a main theme in implantology. Recently, grafting biomolecules onto a Ti surface has attracted more attention due to their direct participation in the osseointegration process around the implant. Semaphorin 3A (Sema3A) is a new proven osteoprotection molecule and is considered to be a promising therapeutic agent in bone diseases, but how to immobilize the protein onto a Ti surface to acquire a long-term effect is poorly defined. In our study, we tried to use chitosan to wrap Sema3A (CS/Sema) and connect to the microarc oxidized Ti surface via silane glutaraldehyde coupling. The microarc oxidization could formulate porous topography on a Ti surface, and the covalently bonded coating was homogeneously covered on the ridges between the pores without significant influence on the original topography. A burst release of Sema3A was observed in the first few days in phosphate-buffered saline and could be maintained for >2 weeks. Coating in phosphate-buffered saline containing lysozyme was similar, but the release rate was much more rapid. The coating did not significantly affect cellular adhesion, viability, or cytoskeleton arrangement, but the osteogenic-related gene expression was dramatically increased and calcium deposition was also abundantly detected. In conclusion, covalent bonding of CS/Sema could strongly improve osteogenic differentiation of osteoblasts and might be applied for Ti implant surface biofunctionalization.
Url:
DOI: 10.2147/IJN.S68895
PubMed: 25336945
PubMed Central: 4200022
Affiliations:
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sortKey="Fang, Kaixiu" sort="Fang, Kaixiu" uniqKey="Fang K" first="Kaixiu" last="Fang">Kaixiu Fang</name><affiliation wicri:level="1"><nlm:aff id="af1-ijn-9-4649">State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an</wicri:regionArea><wicri:noRegion>Xi’an</wicri:noRegion></affiliation></author><author><name sortKey="Song, Wen" sort="Song, Wen" uniqKey="Song W" first="Wen" last="Song">Wen Song</name><affiliation wicri:level="1"><nlm:aff id="af2-ijn-9-4649">State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Military Stomatology, Department of Prosthetic Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an</wicri:regionArea><wicri:noRegion>Xi’an</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Lifeng" sort="Wang, Lifeng" uniqKey="Wang L" first="Lifeng" last="Wang">Lifeng Wang</name><affiliation wicri:level="1"><nlm:aff id="af1-ijn-9-4649">State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an</wicri:regionArea><wicri:noRegion>Xi’an</wicri:noRegion></affiliation></author><author><name sortKey="Jia, Sen" sort="Jia, Sen" uniqKey="Jia S" first="Sen" last="Jia">Sen Jia</name><affiliation wicri:level="1"><nlm:aff id="af3-ijn-9-4649">State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Military Stomatology, Department of Oral and Maxillofacial Surgery, School of Stomatology, Fourth Military Medical University, Xi’an</wicri:regionArea><wicri:noRegion>Xi’an</wicri:noRegion></affiliation></author><author><name sortKey="Wei, Hongbo" sort="Wei, Hongbo" uniqKey="Wei H" first="Hongbo" last="Wei">Hongbo Wei</name><affiliation wicri:level="1"><nlm:aff id="af1-ijn-9-4649">State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an</wicri:regionArea><wicri:noRegion>Xi’an</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Shuai" sort="Ren, Shuai" uniqKey="Ren S" first="Shuai" last="Ren">Shuai Ren</name><affiliation wicri:level="1"><nlm:aff id="af1-ijn-9-4649">State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an</wicri:regionArea><wicri:noRegion>Xi’an</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Xiaoru" sort="Xu, Xiaoru" uniqKey="Xu X" first="Xiaoru" last="Xu">Xiaoru Xu</name><affiliation wicri:level="1"><nlm:aff id="af1-ijn-9-4649">State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an</wicri:regionArea><wicri:noRegion>Xi’an</wicri:noRegion></affiliation></author><author><name sortKey="Song, Yingliang" sort="Song, Yingliang" uniqKey="Song Y" first="Yingliang" last="Song">Yingliang Song</name><affiliation wicri:level="1"><nlm:aff id="af1-ijn-9-4649">State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, Fourth Military Medical University, Xi’an</wicri:regionArea><wicri:noRegion>Xi’an</wicri:noRegion></affiliation></author></analytic><series><title level="j">International Journal of Nanomedicine</title><idno type="ISSN">1176-9114</idno><idno type="eISSN">1178-2013</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Improving osseointegration of extensively used titanium (Ti) implants still remains a main theme in implantology. Recently, grafting biomolecules onto a Ti surface has attracted more attention due to their direct participation in the osseointegration process around the implant. Semaphorin 3A (Sema3A) is a new proven osteoprotection molecule and is considered to be a promising therapeutic agent in bone diseases, but how to immobilize the protein onto a Ti surface to acquire a long-term effect is poorly defined. In our study, we tried to use chitosan to wrap Sema3A (CS/Sema) and connect to the microarc oxidized Ti surface via silane glutaraldehyde coupling. The microarc oxidization could formulate porous topography on a Ti surface, and the covalently bonded coating was homogeneously covered on the ridges between the pores without significant influence on the original topography. A burst release of Sema3A was observed in the first few days in phosphate-buffered saline and could be maintained for >2 weeks. Coating in phosphate-buffered saline containing lysozyme was similar, but the release rate was much more rapid. The coating did not significantly affect cellular adhesion, viability, or cytoskeleton arrangement, but the osteogenic-related gene expression was dramatically increased and calcium deposition was also abundantly detected. In conclusion, covalent bonding of CS/Sema could strongly improve osteogenic differentiation of osteoblasts and might be applied for Ti implant surface biofunctionalization.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Elias, Cn" uniqKey="Elias C">CN Elias</name></author><author><name sortKey="Lima, Jhc" uniqKey="Lima J">JHC Lima</name></author><author><name sortKey="Valiev, R" uniqKey="Valiev R">R Valiev</name></author><author><name sortKey="Meyers, Ma" uniqKey="Meyers M">MA Meyers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Buser, D" uniqKey="Buser D">D Buser</name></author><author><name sortKey="Janner, Sf" uniqKey="Janner S">SF Janner</name></author><author><name sortKey="Wittneben, Jg" uniqKey="Wittneben J">JG Wittneben</name></author><author><name sortKey="Bragger, U" uniqKey="Bragger U">U Bragger</name></author><author><name sortKey="Ramseier, Ca" uniqKey="Ramseier C">CA 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P">PK Chu</name></author><author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dunleavy, Cs" uniqKey="Dunleavy C">CS Dunleavy</name></author><author><name sortKey="Golosnoy, Io" uniqKey="Golosnoy I">IO Golosnoy</name></author><author><name sortKey="Curran, Ja" uniqKey="Curran J">JA Curran</name></author><author><name sortKey="Clyne, Tw" uniqKey="Clyne T">TW Clyne</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, X" uniqKey="Wang X">X Wang</name></author><author><name sortKey="Xi, Z" uniqKey="Xi Z">Z Xi</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z Liu</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L Yang</name></author><author><name sortKey="Cao, Y" uniqKey="Cao Y">Y Cao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, Y" uniqKey="Yuan Y">Y Yuan</name></author><author><name sortKey="Chesnutt, Bm" uniqKey="Chesnutt B">BM Chesnutt</name></author><author><name sortKey="Wright, L" uniqKey="Wright L">L Wright</name></author><author><name sortKey="Haggard, Wo" uniqKey="Haggard W">WO Haggard</name></author><author><name sortKey="Bumgardner, Jd" uniqKey="Bumgardner J">JD Bumgardner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cust Dio, Ca" uniqKey="Cust Dio C">CA Custódio</name></author><author><name sortKey="Alves, Cm" uniqKey="Alves C">CM Alves</name></author><author><name sortKey="Reis, Rl" uniqKey="Reis R">RL Reis</name></author><author><name sortKey="Mano, Jf" uniqKey="Mano J">JF Mano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamilton, V" uniqKey="Hamilton V">V Hamilton</name></author><author><name sortKey="Yuan, Y" uniqKey="Yuan Y">Y Yuan</name></author><author><name sortKey="Rigney, Da" uniqKey="Rigney D">DA Rigney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tran, Ts" uniqKey="Tran T">TS Tran</name></author><author><name sortKey="Kolodkin, Al" uniqKey="Kolodkin A">AL Kolodkin</name></author><author><name sortKey="Bharadwaj, R" uniqKey="Bharadwaj R">R Bharadwaj</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lepelletier, Y" uniqKey="Lepelletier Y">Y Lepelletier</name></author><author><name sortKey="Moura, Ic" uniqKey="Moura I">IC Moura</name></author><author><name sortKey="Hadj Slimane, R" uniqKey="Hadj Slimane R">R Hadj-Slimane</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Fang, Kaixiu" sort="Fang, Kaixiu" uniqKey="Fang K" first="Kaixiu" last="Fang">Kaixiu Fang</name></noRegion><name sortKey="Jia, Sen" sort="Jia, Sen" uniqKey="Jia S" first="Sen" last="Jia">Sen Jia</name><name sortKey="Ren, Shuai" sort="Ren, Shuai" uniqKey="Ren S" first="Shuai" last="Ren">Shuai Ren</name><name sortKey="Song, Wen" sort="Song, Wen" uniqKey="Song W" first="Wen" last="Song">Wen Song</name><name sortKey="Song, Yingliang" sort="Song, Yingliang" uniqKey="Song Y" first="Yingliang" last="Song">Yingliang Song</name><name sortKey="Wang, Lifeng" sort="Wang, Lifeng" uniqKey="Wang L" first="Lifeng" last="Wang">Lifeng Wang</name><name sortKey="Wei, Hongbo" sort="Wei, Hongbo" uniqKey="Wei H" first="Hongbo" last="Wei">Hongbo Wei</name><name sortKey="Xu, Xiaoru" sort="Xu, Xiaoru" uniqKey="Xu X" first="Xiaoru" last="Xu">Xiaoru Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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